Q: Which VitroGel hydrogel product should I choose?

Ready-To-Use Hydrogel For many users, this is the preferred hydrogel system for many applications. It is a ready-to-use hydrogel where you need only to add your cells, cover medium, and incubate. The hydrogels come with different versions of optimized formulations for different cell types and applications: It is a ready-to-use hydrogel where you need only to add your cells, cover medium, and incubate. <ul> <li>VitroGel Hydrogel Matrix (SKU: VHM01): a general-purpose hydrogel that is good for many cell lines and primary cells. This hydrogel can support the formation of tumor spheroid, the cellular network structure of stromal or fibroblast cells, co-culture, invasion or 3D cell migration.</li> <li>VitroGel ORGANOID (SKU: VHM04): supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources.</li> <li>VitroGel STEM (SKU: VHM02): generate high-quality 3D stem cells directly from liquid nitrogen. Excellent for stem cell expansion, 3D scale-up or downstream applications.</li> <li>VitroGel MSC (SKU: VHM03): support 2D hydrogel coating, 3D cultures of mesenchymal stem cells (MSCs) and make hydrogel cell beads to replace microcarrier for MSC scale-up.</li> <li>VitroGel HEK293 (SKU: VHM05): support 3D culture and scale up of human embryonic kidney 293 (HEK293) cells for cell-based bioproduction.</li> <li>VitroGel Angiogenesis Assay Kit (VHM06-K1): a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesi</li> </ul>

Q: Which VitroGel ORGANOID hydrogel should I choose for my organoid type?

<img src="https://storage.crisp.chat/users/helpdesk/website/16e3d682039ef000/vitrogel-organoid-discovery-ki_1f9ol04.png" alt="VitroGel® ORGANOID Discovery Kit" /> The VitroGel® ORGANOID Discovery Kit includes the four different formulation types of VitroGel ORGANOID hydrogels: VitroGel® ORGANOID 1, VitroGel® ORGANOID 2, VitroGel® ORGANOID 3, and VItroGel® ORGANOID 4 Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions. Because there is a wide range of organoid cell resources from stem cells, patient-derived tissue, co-culture, and PDX, it is hard to tell which organoid hydrogel would perform the best for the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for your organoid conditions. From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids. By saying that, we still suggest using the Discovery Kit to make a quick screening to find the hydrogel that is optimal for your organoid conditions.

Question 1

What is VitroGel?

Accepted Answer

VitroGel® is a xeno-free functional hydrogel system that closely mimics the natural extracellular matrix (ECM) environment. Modified with multiple cellular functional ligands, VitroGel allows for a robust 3D cell culture platform and can be used as an injectable delivery system for drug discovery, tissue engineering, cell therapy, and personalized medicine.

VitroGel comes in two different variations, ready-to-use and high concentration.

The ready-to-use VitroGel is a series of user-friendly functional hydrogels offering an excellent balance of simplicity and versatility. The hydrogels have optimized formulations of multi-functional ligands and concentration. The hydrogel solution is stable at room temperature and can mix with cells/culture medium directly to use.

VitroGel High Concentration hydrogels give scientists the full control to adjust both mechanical strength and functional ligands of the cell culture environment. The high concentration formulation allows the maximum flexibility to manipulate the mechanical strength of hydrogel by adjust the dilution ratios of the hydrogel solution. VitroGel High Concentration Hydrogels also can use as a set of building blocks to create a functional micro-environment by blending (“mix and match”) different versions.

Question 2

What is the chemical composition of the VitroGel?

Accepted Answer

The hydrogel is a xeno-free synthetic polysaccharide-based hydrogel system

Question 3

Which VitroGel hydrogel product should I choose?

Accepted Answer

Ready-To-Use Hydrogel

For many users, this is the preferred hydrogel system for many applications. It is a ready-to-use hydrogel where you need only to add your cells, cover medium, and incubate. The hydrogels come with different versions of optimized formulations for different cell types and applications: It is a ready-to-use hydrogel where you need only to add your cells, cover medium, and incubate.

VitroGel Hydrogel Matrix (SKU: VHM01): a general-purpose hydrogel that is good for many cell lines and primary cells. This hydrogel can support the formation of tumor spheroid, the cellular network structure of stromal or fibroblast cells, co-culture, invasion or 3D cell migration.
VitroGel ORGANOID (SKU: VHM04): supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources.
VitroGel STEM (SKU: VHM02): generate high-quality 3D stem cells directly from liquid nitrogen. Excellent for stem cell expansion, 3D scale-up or downstream applications.
VitroGel MSC (SKU: VHM03): support 2D hydrogel coating, 3D cultures of mesenchymal stem cells (MSCs) and make hydrogel cell beads to replace microcarrier for MSC scale-up.
VitroGel HEK293 (SKU: VHM05): support 3D culture and scale up of human embryonic kidney 293 (HEK293) cells for cell-based bioproduction.
VitroGel Angiogenesis Assay Kit (VHM06-K1): a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesi

Question 4

What is the elastic modulus of ready-to-use gels?

Accepted Answer

The elastic modulus of the ready-to-use VitroGel is about 100-300 Pa. Please keep in mind, the elastic modulus (G’) was tested by dynamic rheometer. The G’ is different than stiffness (Young’s modulus). A simple conversion between G’ and stiffness is 1:3 (100 Pa of G’ is about 300 Pa of stiffness). Therefore, the stiffness of ready-to-use VitroGel is about 300-900 Pa.

Question 5

Which VitroGel ORGANOID hydrogel should I choose for my organoid type?

Accepted Answer

VitroGel® ORGANOID Discovery Kit

The VitroGel® ORGANOID Discovery Kit includes the four different formulation types of VitroGel ORGANOID hydrogels: VitroGel® ORGANOID 1, VitroGel® ORGANOID 2, VitroGel® ORGANOID 3, and VItroGel® ORGANOID 4

Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.

Because there is a wide range of organoid cell resources from stem cells, patient-derived tissue, co-culture, and PDX, it is hard to tell which organoid hydrogel would perform the best for the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for your organoid conditions.

From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids. By saying that, we still suggest using the Discovery Kit to make a quick screening to find the hydrogel that is optimal for your organoid conditions.

Question 6

Can I do live/dead assay and cell proliferation assay on VitroGel?

Accepted Answer

Yes. You can use Cyto3D™ Live-Dead Assay Kit to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating and on monolayer.

Please check the product page of Cyto3D™ Live-Dead Assay Kit for more information: https://www.thewellbio.com/product/live-dead-cell-assay-kit-cyto3d/

Frequently Read Articles

Browse All Categories