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# How often does the cover media need to be changed for 3D culture?

Typically, we recommend changing the cover medium every other day, similar to regular 2D cell culture. However, it depends on the experiment’s needs. Some experiments might require changing every 24 hours and some might not require changing for an entire week. Please contact us at [support@thewellbio.com](support@thewellbio.com) if you are unsure of this.