Q: Which VitroGel ORGANOID hydrogel should I choose for my organoid type?

<img src="https://storage.crisp.chat/users/helpdesk/website/16e3d682039ef000/vitrogel-organoid-discovery-ki_1f9ol04.png" alt="VitroGel® ORGANOID Discovery Kit" /> The VitroGel® ORGANOID Discovery Kit includes the four different formulation types of VitroGel ORGANOID hydrogels: VitroGel® ORGANOID 1, VitroGel® ORGANOID 2, VitroGel® ORGANOID 3, and VItroGel® ORGANOID 4 Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions. Because there is a wide range of organoid cell resources from stem cells, patient-derived tissue, co-culture, and PDX, it is hard to tell which organoid hydrogel would perform the best for the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for your organoid conditions. From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids. By saying that, we still suggest using the Discovery Kit to make a quick screening to find the hydrogel that is optimal for your organoid conditions.

Question 1

What is VitroGel?

Accepted Answer

VitroGel® is a xeno-free functional hydrogel system that closely mimics the natural extracellular matrix (ECM) environment. Modified with multiple cellular functional ligands, VitroGel allows for a robust 3D cell culture platform and can be used as an injectable delivery system for drug discovery, tissue engineering, cell therapy, and personalized medicine.

VitroGel comes in two different variations, ready-to-use and high concentration.

The ready-to-use VitroGel is a series of user-friendly functional hydrogels offering an excellent balance of simplicity and versatility. The hydrogels have optimized formulations of multi-functional ligands and concentration. The hydrogel solution is stable at room temperature and can mix with cells/culture medium directly to use.

VitroGel High Concentration hydrogels give scientists the full control to adjust both mechanical strength and functional ligands of the cell culture environment. The high concentration formulation allows the maximum flexibility to manipulate the mechanical strength of hydrogel by adjust the dilution ratios of the hydrogel solution. VitroGel High Concentration Hydrogels also can use as a set of building blocks to create a functional micro-environment by blending (“mix and match”) different versions.

Question 2

What is the elastic modulus of ready-to-use gels?

Accepted Answer

The elastic modulus of the ready-to-use VitroGel is about 100-300 Pa. Please keep in mind, the elastic modulus (G’) was tested by dynamic rheometer. The G’ is different than stiffness (Young’s modulus). A simple conversion between G’ and stiffness is 1:3 (100 Pa of G’ is about 300 Pa of stiffness). Therefore, the stiffness of ready-to-use VitroGel is about 300-900 Pa.

Question 3

Which VitroGel ORGANOID hydrogel should I choose for my organoid type?

Accepted Answer

VitroGel® ORGANOID Discovery Kit

The VitroGel® ORGANOID Discovery Kit includes the four different formulation types of VitroGel ORGANOID hydrogels: VitroGel® ORGANOID 1, VitroGel® ORGANOID 2, VitroGel® ORGANOID 3, and VItroGel® ORGANOID 4

Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.

Because there is a wide range of organoid cell resources from stem cells, patient-derived tissue, co-culture, and PDX, it is hard to tell which organoid hydrogel would perform the best for the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for your organoid conditions.

From our findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids. By saying that, we still suggest using the Discovery Kit to make a quick screening to find the hydrogel that is optimal for your organoid conditions.

Question 4

What is the chemical composition of the VitroGel?

Accepted Answer

The hydrogel is a xeno-free synthetic polysaccharide-based hydrogel system

Question 5

Does VitroGel contain any natural ECM proteins like fibronectin, collagens, or laminins?

Accepted Answer

VitroGel does not contain any natural ECM proteins. Scientists can add growth factors and proteins to the hydrogel system for a well-defined system.

Question 6

Can I do live/dead assay and cell proliferation assay on VitroGel?

Accepted Answer

Yes. You can use Cyto3D™ Live-Dead Assay Kit to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating and on monolayer.

Please check the product page of Cyto3D™ Live-Dead Assay Kit for more information: https://www.thewellbio.com/product/live-dead-cell-assay-kit-cyto3d/

Frequently Read Articles

Browse All Categories