• Can I do live/dead assay and cell proliferation assay on VitroGel?

  Yes. You can use Cyto3D™ Live-Dead Assay Kit to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating and on monolayer. Please check the product page of Cyto3D™ Live-Dead Assay Kit for more information: https://www.thewellbio.com/product/live-dead-cell-assay-kit-cyto3d/Some readers

• Can I do molecular analyses of protein or nucleic acids (DNA/RNA) of cells cultured in VitroGel?

  Yes. Cells can be harvested from the hydrogel and be used for molecular analyses according to standard procedures. The hydrogel is transparent, and you can use the molecular assay directly with the hydrogel. Additionally, the hydrogel can be easily dissolved by using a homogenizer/ultrasonic processor, so scientists can lysis cells together with hydrogel to extract DNA/RNA. Please check the protocols for more details: [https://www.thewellbio.com/protocols/](https://www.thewellbio.com/protocols/Few readers

• Is VitroGel compatible with staining and immunofluorescence protocols for confocal microscopy or other imaging technologies?

  Yes. Cells can be stained within the hydrogel or harvested from the hydrogel and then stain. Most fluorescent dyes and immunological reagents can be used at standard protocols. VitroGel hydrogel is transparent and compatible with different imaging systems for cell observation. Please check the protocols for more details: https://www.thewellbio.com/protocols/Few readers

• Is it possible to fix cells inside the hydrogel with PFA and performed immunolabelling?

  Yes. The cells can be fixed inside the hydrogel with PFA. Immunolabeling of cells works well with VitroGel.Few readers